脱氧雪腐镰刀菌烯醇暴露对断奶仔猪延髓脂质过氧化反应、神经递质及钙稳态的影响

doi:10.11843/j.issn.0366-6964.2019.05.020

摘要/Abstract

摘要：

旨在通过脱氧雪腐镰刀菌烯醇（DON）体内暴露试验，检测仔猪延髓组织的脂质过氧化反应、神经递质分泌及钙稳态的变化，研究DON对仔猪的神经毒性作用。选取30头21日龄"杜×长×大"三元杂交断奶仔猪，随机分为对照组、DON低剂量组和DON高剂量组，每组10头仔猪。对照组饲喂基础饲料，DON低剂量组和高剂量组分别饲喂含1、2 mg·kg^-1DON的饲料，试验期为60 d。试验结束时，每组随机选择5头仔猪屠宰，采集延髓组织，检测氧化与过氧化水平、神经递质含量和Ca²⁺浓度、钙调蛋白（CaM）与钙调蛋白依赖性激酶Ⅱ（CaMKⅡ）mRNA相对表达量及CaM、CaMKⅡ、p-CaMKⅡ的蛋白表达水平。结果显示：与对照组相比，DON高、低剂量组脂质过氧化指标超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性极显著降低（P<0.01），而一氧化氮（NO）浓度极显著升高（P<0.01）；DON高、低剂量组神经递质5-羟色胺（5-HT）浓度极显著升高（P<0.01），而乙酰胆碱（ACH）浓度极显著降低（P<0.01），高剂量组多巴胺（DA）和γ-氨基丁酸（GABA）浓度显著下降（P<0.05）；DON高、低剂量组钙稳态指标Ca²⁺浓度均极显著升高（P<0.01），低剂量组CaM mRNA表达量显著降低（P<0.05），高、低剂量组CaMKⅡ mRNA表达量均极显著降低（P<0.01）；DON高剂量组CaM蛋白表达水平极显著降低（P<0.01），而p-CaMKⅡ蛋白表达水平极显著升高（P<0.01）。试验结果表明，DON暴露能够改变仔猪延髓组织脂质过氧化反应及神经递质的分泌，并导致钙稳态紊乱，对断奶仔猪具有一定的神经毒性作用。

Abstract:

This experiment was conducted to investigate the nerve toxicological effects of medulla oblongata tissues induced by deoxynivalenol (DON), through the changes of lipid peroxidation, neurotransmitter secretion and calcium homeostasis. Thirty weaned piglets (21 days old) were randomly divided into control group, low-dose and high-dose DON groups, with 10 piglets in each group. The piglets in control group were fed with basal diet, and DON low-dose group and high-dose group were fed with DON diet containing 1 and 2 mg·kg^-1 DON, respectively. The trial was conducted for 60 days. At the end of the experiment, five piglets were randomly selected from each group for slaughter. The medulla oblongata tissue was collected to detect the activities of oxidation and antioxidation indexes, neurotransmitter and Ca²⁺ concentrations, the relative expression of calmodulin (CaM) and calmodulin-dependent kinase Ⅱ (CaMK Ⅱ) mRNA, and the protein expression levels of CaM, CaMKⅡ and p-CaMKⅡ were measured. Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in medulla oblongata of piglets in high and low doses of DON group decreased significantly (P<0.01), while the concentration of nitric oxide (NO) increased significantly (P<0.01). The concentration of serotonin (5-HT) in high and low doses of DON group were significantly increased (P<0.01),while the concentration of acetylcholine (ACH) was significantly decreased (P<0.01). The concentration of dopamine (DA) and gamma-aminobutyric acid (GABA) in high doses of DON group were significantly decreased (P<0.05).The concentrations of Ca²⁺ in high and low doses of DON group were significantly increased (P<0.01).The relative expression of CaM mRNA in low doses of DON group were significantly decreased (P<0.05), the expression of CaMKⅡ mRNA in high and low doses of DON group were significantly decreased (P<0.01),the expression level of CaM protein in high doses of DON group was significantly decreased (P<0.01), while the expression of p-CaMKⅡ protein was significantly increased (P<0.01). These results suggest that DON exposure can change the lipid peroxidation reaction and the secretions of neurotransmitters and neuronal calcium homeostasis in medulla oblongata tissues of weaned piglets. It suggests that DON has some neurotoxic effects in piglets.

中图分类号:

S856.9

储小燕, 朱雷, 曹利, 陈晓芳, 李玉, 冯士彬, 吴金节, 王希春. 脱氧雪腐镰刀菌烯醇暴露对断奶仔猪延髓脂质过氧化反应、神经递质及钙稳态的影响[J]. 畜牧兽医学报, 2019, 50(5): 1091-1098.

CHU Xiaoyan, ZHU Lei, CAO Li, CHEN Xiaofang, LI Yu, FENG Shibin, WU Jinjie, WANG Xichun. Effects of Deoxynivalenol Exposure on Lipid Peroxidation, Neurotransmitters and Calcium Homeostasis in the Medulla Oblongata of Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1091-1098.

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